Primer Annealing Temperature Calculator For PCR, Decreasing The Length Of A Primer, Will Likely Lead Me To:?

For PCR, decreasing the length of a primer, will likely lead me to:? - primer annealing temperature calculator

A. Increase the denaturation step time
B. Denaturing Cut no time
C. The reduction in the annealing temperature
D. Increase the extension
E. Increase the number of cycles

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